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caco 2 monolayer  (World Precision Instruments)


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    World Precision Instruments caco 2 monolayer
    Caco 2 Monolayer, supplied by World Precision Instruments, used in various techniques. Bioz Stars score: 99/100, based on 221 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/caco 2 monolayer/product/World Precision Instruments
    Average 99 stars, based on 221 article reviews
    caco 2 monolayer - by Bioz Stars, 2026-02
    99/100 stars

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    Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Journal: Frontiers in Immunology

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    doi: 10.3389/fimmu.2025.1671729

    Figure Lengend Snippet: Effects of LGGp on gut barrier integrity. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) The TEER values were measured as follows: TEER = (measured resistance value−blank value) × single cell layer surface area (cm 2 ). The exposure to LGGp elicited a significant increase in TEER (A) . Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 h (B, C, D). FITC dextran permeability was assessed in transwell plate (B) and appeared reduced after 2 h in cells pre-treated with LGGp. Cells were processed for mRNA analysis by RT-PCR. Occludin (C) and ZO-1 (D) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucoronidase beta (GUS-B). Each point represents median and error with range (A, B) or median and interquartile range (C, D) of five independent experiments. Data were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; TEER, Trans-epithelial electrical resistance; ZO-1, zonula occludens 1. *p<0.05 vs NT.

    Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

    Techniques: Permeability, Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY

    Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Journal: Frontiers in Immunology

    Article Title: Postbiotic effects elicited by heat-inactivated Lacticaseibacillus rhamnosus GG against cow’s milk allergy in human cells

    doi: 10.3389/fimmu.2025.1671729

    Figure Lengend Snippet: Effects of LGGp on human enterocytes differentiation. Caco-2 cells were stimulated with 10 µg/ml LGGp for 48 (h) Cells were processed for mRNA analysis by RT-PCR. Muc2 (A) and Lactase (B) expression levels were significantly increased in Caco-2 cells exposed to LGGp. RT-PCR analysis was performed using the comparative threshold cycle (CT) method. Gene expression was normalized against the expression of the reference gene glucuronidase beta (GUS-B). Each point represents median and interquartile range of five independent experiments. Data were analyzed were analyzed using Mann Whitney U test. LGGp, heat-inactivated LGG postbiotic; Muc-2, mucin 2; *p<0.05 vs NT.

    Article Snippet: For all experiments, we used a well validated model of gut barrier based on Caco-2 cells monolayer (American Type Culture Collection, Middlesex, UK; accession number: HTB-37) ( , ).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Gene Expression, MANN-WHITNEY